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Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of muta(trade mark)mouse lung epithelial cells

Jacobsen NR, Saber AT, White P, et al. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of muta(trade mark)mouse lung epithelial cells. Environ Mol Mutagen 2007;48(6):451-61.
Date: 2007
Type: Scientific Article
Abstract: Carbon black and quartz are relatively inert solid particulate materials that are carcinogenic in laboratory animals. Quartz is a human carcinogen, whereas data on carbon black are contradictory, and there are few data on mammalian mutagenesis. We determined the mutant frequency following eight repeated 72-hr incubations with 75 g/ml carbon black (Printex 90) or 100 g/ml quartz (SRM1878a) particles in the FE1 MutaTMMouse lung epithelial cell line. For carbon black exposed cells, the mutant frequency was 1.40-fold (95% CI: 1.22-1.58) for cII and 1.23-fold (95% CI: 1.10-1.37) for lacZ compared with identically passaged untreated cells. Quartz did not significantly affect the mutant frequency. Carbon black also induced DNA strand breaks (P = 0.02) and oxidized purines (P = 0.008), as measured by the Comet assay. Quartz induced marginally more oxidized purines, but no change in strand breaks. We detected five (phenanthrene, flouranthene, pyrene, benzo[a]anthracene, and chrysene) of the 16 EPA priority polycyclic aromatic hydrocarbons (PAHs) in an extract of carbon black. The detected PAHs are only weakly mutagenic compared with benzo[a]pyrene, and were present in very low amounts. In conclusion, carbon black was weakly mutagenic in vitro at the cII and lacZ loci. It also induced DNA strand breaks and oxidized DNA bases. More studies are essential for understanding the biological significance of these findings, and clearly documenting DNA sequence changes. The results do not necessarily imply that other carbonaceous nano materials are genotoxic.
Link: http://dx.doi.org/10.1002/em.20300
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Created: 01.08.2007
 
Updated  01.08.2007
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